6D- Plant Substances as Potential Medicines
Purpose: To find active plant ingredients found around Novato that can inhibit bacterial growth.
Materials:
LB broth Media bottles Sterilizer Hot water bath LB agar Laminar flow hood Disinfectant Safety glasses Bunsen burner Inoculating loop(Ni/Cr wire) Petri dishes Incubator oven Tweezers Alcohol |
Stock plate Plant sample Mortar and pestle Pipet(10 mL, 1 mL) Short-stemmed plastic funnels Filter paper discs(5 mm) Beakers(100 mL) Syringe and filter Reaction tubes Tube racks Methanol Heat block Dry block heater Ampicillin Glass spreader |
Procedure Part 1: First, the mortar and pestle is used to grind two grams of plant tissue and combine it with ten milliliters of deionized water. The solution rests for three minutes before filtering the sample through a filter paper funnel. Then, a syringe filter is used to extract the filtered sample to sterilize it. A one milliliter sample of the extract is transferred to a microtube and labelled. These steps are repeat with methanol in place of water to extract different components. Let the extract sit on heat blocks for twenty-four hours to evaporate the methanol.